Atomic force microscopy analysis of rolling circle amplification of plasmid DNA
نویسندگان
چکیده
منابع مشابه
Direct retransformation of yeast with plasmid DNA isolated from single yeast colonies using rolling circle amplification.
We have efficiently amplified plasmid DNA from single yeast colonies using rolling circle amplification (RCA). The amplified DNA can be directly used for restriction digestion, DNA sequencing, or yeast transformation. The RCA-based high-fidelity amplification would be useful for plasmid manipulation in a variety of yeast-based systems, particularly for high-throughput analyses.
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Since its recent implementation at one of the world's largest high-throughput sequencing centers, the utility of MP-RCA for DNA sequencing has been thoroughly validated. However, applications of this technology extend far beyond DNA sequencing. While many of these applications have been explored in this chapter, the future will undoubtedly add to this growing list.
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ژورنال
عنوان ژورنال: Archives of Histology and Cytology
سال: 2003
ISSN: 0914-9465,1349-1717
DOI: 10.1679/aohc.66.175